The present study describes selected issues associated with the emission level in toxic exhaust gases and fuel injection timing. The study was focused on the following types of fuels: Diesel oil (the base fuel) and the other fuels were the mixture of fatty acid methyl ester with Camelina (L10 – diesel fuel with 10% V/V FAME of Camelina and L20 – diesel fuel with 10% V/V FAME of Camelina) was used. Fuel injection advanced angle was set for three different values – the factory setting – 12° before TDC, later injection – 7° and earlier injection – 17°. The most important conclusion is that in most measurement points registered in the same engine operating conditions, the concentration of fuel NOx in L10 and L20 increased but PM emissions decreased which is caused by active oxygen located in the internal structure of the fuel. This fact contributes to the rise in temperature during the combustion process. At the same time factory settings of the angle makes NOx emissions lower and close to reference fuel.